Hi, laboratory technician of the BGM group, I work at the intersection of modern molecular biology and with classical biochemistry to engineer enzyme variants and study their properties.
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From: Belgium
Background: Bachelor in Chemistry, Field of engineering and Technology, HE VINCI Paul Lambin, Bruxelles BE
Projects:
Conversion of a D-Alanyl-D-Alanine peptidase into a β -lactamase.
DD-peptidases, also called PBPs (Penicillin Binding Proteins), are part of an enzyme family involved in the synthesis and modification of the peptidoglycan of the bacterial cell wall. β-lactam antibiotics target these PBPs by forming stable acylenzymes, which inhibit them in a permanent manner. This inhibition kills the bacteria. Some bacteria have acquired β-lactam resistance by expressing enzymes called β-lactamases that hydrolyses antibiotics before they reach their target. β -lactamases have likely emerged during evolution from PBP-like ancestors.
Although the conversion of a PBP into a beta-lactamase seems a priori straightforward, it has proven to be extremely difficult in the lab.
Contact:
UCLouvain/LIBST/BGM
Croix du sud 4-5/L7.07.06
Carnoy b.247
1348 Louvain-la-Neuve
Damien.evrarD@uclouvain.be
UClouvain directory
Phone: +32(0)10 47 26 32
https://orcid.org/0000-0002-1521-0444
” A person who never made a mistake never tried anything new “
Albert Einstein