Selected Journal Article

Below are selected articles displaying the laboratory’s current research interest. A full list of publication can be found on the website of the Université catholique de Louvain.

2021

Godessart, Pierre ; Lannoy, Adélie ; Dieu, Marc ; Van der Verren, Sander E ; Soumillion, Patrice ; et. al. β-Barrels covalently link peptidoglycan and the outer membrane in the α-proteobacterium Brucella abortus.. In: Nature microbiology, Vol. 6, no.1, p. 27-33 (2021)

2020

Yang, Jinsung ; Petitjean, Simon J. L. ; Koehler, Melanie ; Zhang, Qingrong ; Dumitru, Andra C. ; et. al. Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor. In: Nature Communications, Vol. 11, no.1, p. 4541 (2020)

http://hdl.handle.net/2078.1/238509

Dorrazehi, Gol Mohammad ; Worms, Sebastian ; Chirakadavil, Jason Baby ; Mignolet, Johann ; Hols, Pascal ; et. al. Building scarless gene libraries in the chromosome of bacteria. In: Iranzo, Olga, Roque, Ana Cecília, Springer Protocols – Peptide and Protein Engineering, From Concepts to Biotechnological Applications,  2020

http://hdl.handle.net/2078.1/231618

Gargiulo, Serena ; Soumillion, Patrice. Directed evolution for enzyme development in biocatalysis.. In: Current opinion in chemical biology, Vol. 61, p. 107-113 (2020)

http://hdl.handle.net/2078.1/243893

Khan, Mohammad Shahneawz ; Gargiulo, Serena ; Soumillion, Patrice. Promiscuous activity of 3-isopropylmalate dehydrogenase produced at physiological level affords Escherichia coli growth on d-malate.. In: FEBS letters, (2020)

http://hdl.handle.net/2078.1/231614

Desguin, Benoît ; Urdiain-Arraiza, Julian ; Da Costa, Matthieu ; Fellner, Matthias ; Hu, Jian ; et. al. Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases.. In: Scientific reports, Vol. 10, no.1, p. 18123 (2020)

http://hdl.handle.net/2078.1/241914

2019

van den Biggelaar, Ludivine ; Soumillion, Patrice ; Debecker, Damien P.. Biocatalytic transamination in a monolithic flow reactor: improving enzyme grafting for enhanced performance. In: RSC Advances, Vol. 9, no.32, p. 18538-18546 (2019)

http://hdl.handle.net/2078.1/216580

2018

Crowet, Jean Marc ; Nasir, Mehmet Nail ; Dony, Nicolas ; Deschamps, Antoine ; Stroobant, Vincent ; et. al. Insight into the Self-Assembling Properties of Peptergents: A Molecular Dynamics Simulation Study.. In: International Journal of Molecular Sciences (Print), Vol. 19, no.9, p. 2772 (2018)

http://hdl.handle.net/2078.1/203722

Desguin, Benoît ; Fellner, Matthias ; Riant, Olivier ; Hu, Jian ; Hausinger, Robert ; Hols, Pascal ; Soumillion, Patrice. Biosynthesis of the nickel-pincer nucleotide cofactor of lactate racemase requires a CTP-dependent cyclometallase.. In: Journal of Biological Chemistry, (2018). doi:10.1074/jbc.RA118.003741.

http://hdl.handle.net/2078.1/199517

2017

van den Biggelaar, Ludivine ; Soumillion, Patrice ; Debecker, Damien P.. Enantioselective Transamination in Continuous Flow Mode with Transaminase Immobilized in a Macrocellular Silica Monolith. In: Catalysts, Vol. 7, no.54, p. 1-13 (2017). doi:10.3390/catal7020054.

http://hdl.handle.net/2078.1/182359

Galka, Pierre ; Jamez, Elisabeth ; Joachim, Gilles ; Soumillion, Patrice. QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides.. In: PLoS One, Vol. 12, p. e0175146 (2017). doi:10.1371/journal.pone.0175146 (Soumis).

http://hdl.handle.net/2078.1/192409

Galka, Pierre ; Jamez, Elisabeth ; Joachim, Gilles ; Soumillion, Patrice. QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides.. In: PLoS One, Vol. 12, no.4, p. e0175146 (2017). doi:10.1371/journal.pone.0175146.

http://hdl.handle.net/2078.1/185861

Desguin, Benoît ; Soumillion, Patrice ; Hausinger, Robert P. ; Hols, Pascal. Unexpected complexity in the lactate racemization system of lactic acid bacteria. In: FEMS Microbiology Reviews, Vol. 41, no. Supp_1, p. S71-S83 (2017 Aug 1). doi:10.1093/femsre/fux021.

http://hdl.handle.net/2078.1/185722

2016

Desguin, Benoît ; Soumillion, Patrice ; Hols, Pascal ; Hausinger, Robert P. Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion.. In: Proceedings of the National academy of sciences of the United States of America, Vol. 113, no.20, p. 5598-5603 (2016). doi:10.1073/pnas.1600486113.

http://hdl.handle.net/2078.1/180443

2015

Desguin, Benoît ; Zhang, Tuo ; Soumillion, Patrice ; Hols, Pascal ; Hu, Jian ; Hausinger, Robert P. A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase.. In: Science, Vol. 349, no.6243, p. 66-69 (2015). doi:10.1126/science.aab2272.

http://hdl.handle.net/2078.1/162199

Woronoff, Gabrielle ; Ryckelynck, Michaël ; Wessel, Julia ; Schicke, Olivier ; Griffiths, Andrew D ; Soumillion, Patrice. Activity-Fed Translation (AFT) Assay: A New High-Throughput Screening Strategy for Enzymes in Droplets.. In: ChemBioChem : a European journal of chemical biology, Vol. 16, no. 9, p. 1343-1349 (2015). doi:10.1002/cbic.201500087.

http://hdl.handle.net/2078.1/162036

Proost, Joris ; Deschuyteneer, Geneviève ; Santoro, Ronny ; Van Overmeere, Quentin ; Soumillion, Patrice ; Flandre, Denis. Filamentous Phages Displaying Multivalent Peptide Motives With Specific Affinity To Anodic Alumina Surfaces. In: Journal of Biosensors & Bioelectronics, Vol. 6, no. 1, p. 1000162 (2015). doi:10.4172/2155-6210.1000162.

http://hdl.handle.net/2078.1/159065

2014

Vorobieva, Anastassia A ; Khan, Mohammad Shahneawz ; Soumillion, Patrice. Escherichia coli D-malate dehydrogenase, a generalist enzyme active in the leucine biosynthesis pathway.. In: Journal of Biological Chemistry, Vol. 289, no.42, p. 29086-29096 (2014). doi:10.1074/jbc.M114.595363.

http://hdl.handle.net/2078.1/156157

Desguin, Benoît ; Goffin, Philippe ; Viaene, Eric ; Kleerebezem, Michiel ; Martin-Diaconescu, Vlad ; Maroney, Michael J ; Declercq, Jean-Paul ; Soumillion, Patrice ; Hols, Pascal. Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system.. In: Nature Communications, Vol. 5, p. 3615 (2014). doi:10.1038/ncomms4615.

http://hdl.handle.net/2078.1/156031

2013

Alsteens, David ; Trabelsi, Heykel ; Soumillion, Patrice ; Dufrêne, Yves. Multiparametric atomic force microscopy imaging of single bacteriophages extruding from living bacteria. In: Nature Communications, Vol. 4, p. 1-7 (2013). doi:10.1038/ncomms3926.

http://hdl.handle.net/2078.1/135929

2012

Ponsard, Isabelle ; Soumillion, Patrice. New immunoenzymatic strategy for rapid and selective growth of salmonella.. In: Journal of clinical microbiology, Vol. 50, p. 4138-40 (2012). doi:10.1128/JCM.01765-12.

http://hdl.handle.net/2078.1/117045

2011

Volkov, Alexander N ; Barrios, Humberto ; Mathonet, Pascale ; Evrard, Christine ; Ubbink, Marcellus ; Declercq, Jean-Paul ; Soumillion, Patrice ; Fastrez, Jacques. Engineering an allosteric binding site for aminoglycosides into TEM1-β-Lactamase.. In: ChemBioChem : a European journal of chemical biology, Vol. 12, no. 6, p. 904-13 (2011). doi:10.1002/cbic.201000568.

http://hdl.handle.net/2078.1/108346

Woronoff, Gabrielle ; El Harrak, Abdeslam ; Mayot, Estelle ; Schicke, Olivier ; Miller, Oliver J ; Soumillion, Patrice ; Griffiths, Andrew D ; Ryckelynck, Michael. New generation of amino coumarin methyl sulfonate-based fluorogenic substrates for amidase assays in droplet-based microfluidic applications.. In: Analytical Chemistry, Vol. 83, no. 8, p. 2852-2857 (2011). doi:10.1021/ac200373n.

http://hdl.handle.net/2078.1/162038

2010

Shi, Yi-Feng ; Soumillion, Patrice ; Ueda, Mitsuyoshi. Effects of catalytic site mutations on active expression of phage fused penicillin acylase. In: Journal of Biotechnology, Vol. 145, no. 2, p. 139-142 (2010). doi:10.1016/j.jbiotec.2009.11.009.

http://hdl.handle.net/2078.1/34161

Mathieu, Valéry ; Fastrez, Jacques ; Soumillion, Patrice. Engineering allosteric regulation into the hinge region of a circularly permuted TEM-1 {beta}-lactamase.. In: Protein engineering, design & selection : PEDS, Vol. 23, no. 9, p. 699-709 (2010). doi:10.1093/protein/gzq041.

http://hdl.handle.net/2078.1/32809

Deschuyteneer, Geneviève ; Garcia, Stephanie ; Michiels, Benjamin ; Baudoux, Bruno ; Degand, Hervé ; Morsomme, Pierre ; Soumillion, Patrice. Intein-Mediated Cyclization of Randomized Peptides in the Periplasm of Escherichia coli and Their Extracellular Secretion. In: ACS Chemical Biology, Vol. 5, no. 7, p. 691-700 (2010). doi:10.1021/cb100072u.

http://hdl.handle.net/2078.1/33700

2020

Dorrazehi, Gol Mohammad ; Worms, Sebastian ; Chirakadavil, Jason Baby ; Mignolet, Johann ; Hols, Pascal ; et. al. Building scarless gene libraries in the chromosome of bacteria. In: Iranzo, Olga, Roque, Ana Cecília, Springer Protocols – Peptide and Protein Engineering, From Concepts to Biotechnological Applications,  2020

http://hdl.handle.net/2078.1/231618

Urvoas, Agathe ; Minard, Philippe ; Soumillion, Patrice. Phage display methodologies. In: Iranzo, Olga, Roque, Ana Cecília, Springer Protocols – Peptide and Protein Engineering, From Concepts to Biotechnological Applications,  2020

http://hdl.handle.net/2078.1/231617

2017

Desguin, Benoît ; Soumillion, Patrice ; Hols, Pascal ; Hu, Jian ; Hausinger, Robert P.. Lactate Racemase and Its Niacin-Derived, Covalently-Tethered, Nickel Cofactor. In: Deborah Zamble, Magdalena Rowińska-Żyrek, Henryk Kozłowski, The Biological Chemistry of Nickel (Metallobiology Series; 10), The Royal Society of Chemistry, 2017. 978-1-78262-498-1. doi:10.1039/9781788010580.

http://hdl.handle.net/2078.1/185720