Laboratory of Neural Differentiation (NEDI)
Last update :
August 2024
Phenotypic analyses of the Central Nervous System
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- immunolabelings on cryosections or whole-mount samples
- in situ hybridization on cryosections or whole-mount samples
- anterograde or retrograde labelings (DiI, Dextran, FluoroGold)
- regular fluorescence, confocal or light-sheet microscopy
- quantitative analysis of neuronal distribution in the spinal cord
Genetics and biochemical analyses
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- quantification of gene expression by RT-qPCR
- chromatin immunoprecipitation
- transient transfection of immortalized cell line
- isolation of spinal neuronal populations by FACS
Chicken embryo electroporation and organotypic cultures
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- chicken embryonic encephalon or spinal cord electroporation
- organotypic culture of embryonic spinal cord explants
- time-lapse imaging of spinal cord slices
Advanced techniques in molecular biology
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- Gateway technology and homologous recombination in bacteria
- manipulation and modification of Bacterial Artificial Chromosomes (BAC)
- recombination-mediated cassette exchange at the Rosa26 locus
Chicken embryonic spinal cord electroporation
Technical expertise
Animal models of adult spinal cord lesions
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- adult spinal cord injury : compression model
- genetic mouse models of Amyotrophic Lateral Sclerosis
