Aims

The aim is to learn the basic techniques of cell biology:
-cell culture and propagation ;
-morphological and microscopic examination of the cells ;
-analysis of cell proliferation and cell survival,
-introduction to the analysis of cell populations by FACS (Fuorescence-Associated Cell Sorter).
Our aim is also to train students to write a laboratory notebook and a training course report.

Main themes

-culture of cell lines in sterile conditions ;
-cell analysis by fluorescent microscopy ;
-biochemical assays (proteins, cell proliferation and cell survival assays) ;
-introduction to FACS analysis and study of the characteristic profile of selected cell populations.

Content and teaching methods

This training course takes place during 5 consecutive afternoons in two research laboratories of the Faculty. Students will work in small groups (usually 2 students) under the supervision of a research scientist.

Other information (prerequisite, evaluation (assessment methods), course materials recommended readings, ...)

Prérequisites : those of Bac2.
Links : This training course requires knowledges from the practical courses of biology (Bac1, MD1107, General Biology) and prepares to the further laboratory training courses (SBIM9212, Stage de laboratoire)
Assessment : continuous and on the basis of the training course report.
Supervision : an assistant (with the help of other members of the research group).