Teacher(s)

De Plaen Etienne; Octave Jean-Noël coordinator;

Language

French

Prerequisites

The prerequisite(s) for this Teaching Unit (Unité d’enseignement – UE) for the programmes/courses that offer this Teaching Unit are specified at the end of this sheet.

Main themes

Cloning and expression tools in prokaryotic and eukaryotic cells. Genetic engineering and production of proteins useful in biomedical sciences. Analysis and modifications of the DNA. High throughput sequencing. Transgenic animals. Gene therapy.

Aims

At the end of this learning unit, the student is able to :
1	The objectives are the learning of molecular biological approaches needed for the analysis and use of genetic material for diagnostic purposes, or for creating tools that are increasingly indispensable in biomedical sciences.

The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.

Content

Description and use of different cloning and expression vectors in prokaryotes. DNA analysis: restriction enzymes, PCR, sequencing. Cloning and expression of cDNA in different cell types. Overexpression and invalidation of genes in transgenic animals. Examples of gene therapy.

Evaluation methods

Written examination: questions with short open answers.

Other information

Lecture notes and reference book: Biotechnology, DP Clark and NJ Pazdernik (Academic Cell).

Bibliography

• Notes de cours et dossier power point.

Teaching materials

• Notes de cours et dossier power point.

Faculty or entity

SBIM