Teacher(s)

De Plaen Etienne; Octave Jean-Noël coordinator;

Language

French

Prerequisites

The prerequisite(s) for this Teaching Unit (Unité d’enseignement – UE) for the programmes/courses that offer this Teaching Unit are specified at the end of this sheet.

Main themes

Cloning of cDNA. Expression of proteins in bacteria. Purification of proteins. Analysis of enzymatic activity.

Aims

At the end of this learning unit, the student is able to :
1	The objectives are the learning of cloning techniques, expression and purification of recombinant proteins, as well as the analysis of their enzymatic activity.

The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.

Content

Subcloning a cDNA from a TOPO vector to a bacterial expression vector. Transformation of bacteria. Production of a protein by genetic engineering. Purification of this protein by affinity chromatography. Protein detection by Western blotting. Analysis of the enzymatic activity of the purified protein. The teachers explain the tools needed to perform the different experiences before the students supervised by the assistants put them into practice.

Evaluation methods

Short written exams before starting the experiments. Evaluation of an individual report describing the manipulations carried out, the results obtained and their interpretation.

Bibliography

• Notes de cours et travaux pratiques.
• Written notes

Teaching materials

• Notes de cours et travaux pratiques.
• Written notes

Faculty or entity

FASB