Histology of the investigated tissues needs to be known.
The prerequisite(s) for this Teaching Unit (Unité d’enseignement – UE) for the programmes/courses that offer this Teaching Unit are specified at the end of this sheet.
Theory about morphological methods used to investigate biological methods
and practical training to address an exemplative problem in one week
· To gain theoretical and practical knowledge of histological and immunohistological techniques, and theoretical notions of in situ hybridization.
· To solve a biological problem by using morphological methods.
The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.
Evaluation will be made by oral examination with presentation of the histological section at the microscope and discussion about the report.
· Theory :
Histological techniques : frozen sections, paraffin sections, synthetic resin embedding
Immunohistochemical techniques
In situ hybridization
· Practical :
Mouse dissection and tissues sampling
Preparation of buffers and fixative solutions
Tissue freezing or fixation in formalin
Frozen sections (demonstration)
Immunohistochemistry on frozen sections (demonstration)
Paraffin embedding
Paraffin sections
H&E and PAS staining
Immunohistochemistry on paraffin sections
Supervised microscopic analysis (video screening)
Students will be supervised by PhD students during the training.
Students will have to make a report of the study, resembling a scientific article. They will be paired for this report (one student investigating the control animal, the other one the test animal).
