Gol Dorrazehi, auteur sur Soumillion Laboratory https://sites.uclouvain.be/soumillionlab/author/gol/ Evolution. Directed. Tue, 28 Jul 2020 08:30:34 +0000 en-US hourly 1 https://sites.uclouvain.be/soumillionlab/wp-content/uploads/2018/10/cropped-Test-1-32x32.png Gol Dorrazehi, auteur sur Soumillion Laboratory https://sites.uclouvain.be/soumillionlab/author/gol/ 32 32 Chromosomal Libraries Chapter is out! https://sites.uclouvain.be/soumillionlab/2020/07/27/chromosomal-libraries/ https://sites.uclouvain.be/soumillionlab/2020/07/27/chromosomal-libraries/#respond Mon, 27 Jul 2020 10:23:19 +0000 https://sites.uclouvain.be/soumillionlab/?p=8403 A lot of the work we do in the laboratory is trying to find better ways to do Directed Evolution. One of the thing we believe is that expressing libraries in their native genomic context let you navigate the fitness

L’article Chromosomal Libraries Chapter is out! est apparu en premier sur Soumillion Laboratory.

]]> A lot of the work we do in the laboratory is trying to find better ways to do Directed Evolution. One of the thing we believe is that expressing libraries in their native genomic context let you navigate the fitness landscape better.

If you want to find out how exactly we build those libraries, the detailed protocols we use, both in E. coli and in S. thermophilus have now been published as a book chapter by Springer Protocols.

“Dorrazehi G.M., Worms S., Chirakadavil J.B., Mignolet J., Hols P., Soumillion P. (2020) Building Scarless Gene Libraries in the Chromosome of Bacteria. In: Iranzo O., Roque A. (eds) Peptide and Protein Engineering. Springer Protocols Handbooks. Humana, New York, NY

Open access:
https://dial.uclouvain.be/pr/boreal/object/boreal:231618

L’article Chromosomal Libraries Chapter is out! est apparu en premier sur Soumillion Laboratory.

]]> https://sites.uclouvain.be/soumillionlab/2020/07/27/chromosomal-libraries/feed/ 0 Journal Club S01E03 https://sites.uclouvain.be/soumillionlab/2019/02/27/journal-club-s01e03/ https://sites.uclouvain.be/soumillionlab/2019/02/27/journal-club-s01e03/#respond Wed, 27 Feb 2019 14:14:21 +0000 https://sites.uclouvain.be/soumillionlab/?p=5023 Discussion on Molecular Causes of Evolvability An interesting review on “The causes of evolvability and their evolution”, published by Payne and Wanger (2018), is discussed. Three major categories on the research of evolvability are discussed: phenotypic heterogeneity, mutational robustness and

L’article Journal Club S01E03 est apparu en premier sur Soumillion Laboratory.

]]> Discussion on Molecular Causes of Evolvability

An interesting review on “The causes of evolvability and their evolution”, published by Payne and Wanger (2018), is discussed. Three major categories on the research of evolvability are discussed: phenotypic heterogeneity, mutational robustness and adaptive landscape topography:

Phenotypic heterogeneity caused by non-genetic mechanisms are discussed:

Robustness against mutations is allowing phenotypes to tolerate more genetic diversification without any change in final behaviour:

The location of an individual or a population on an adaptive landscape, determine the degree of evolvability towards the highest adaptation:

Causes of evolvability highlighted here — phenotypic heterogeneity, mutational robustness and adaptive landscapes — are themselves subject to evolutionary change:

Leave your comments about molecular causes of evolvability and its impact on the traditional dogma of “Evolution”:

L’article Journal Club S01E03 est apparu en premier sur Soumillion Laboratory.

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