Discovery of a new cargo of the Endophilin A3-mediated endocytosis pathway

N-BAR and F-BAR proteins—endophilin-A3 and PSTPIP1—control clathrin-independent endocytosis of L1CAM

In the current study, we focus on the endocytosis of L1CAM. This glycoprotein plays a major role in the development of the nervous system, and is involved in cancer development and is associated with metastases and poor prognosis. First, we demonstrated in our cellular context that L1CAM is mainly a clathrin-independent cargo. Second, the mechanism of L1CAM endocytosis is specifically mediated by the N-BAR domain protein endophilin-A3. Third, we discovered PSTPIP1, an F-BAR domain protein, as a novel actor in this endocytic process. This work was mainly conducted by Camille Lemaigre in collaboration with Institut Curie, University of Lund, Galecto and UNamur.

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