Congratulations to Antoine for his PhD thesis

This week Antoine presented us his elegant work on GDT1-mediated proton transport.

In this thesis, a heterologous expression system using Lactococcus lactis was set up for direct H+ transport assay and a Golgi-localized pH sensor was developed for in vivo measurements in S. cerevisiae. Thanks to them, Gdt1p-mediated H+ transport activity was demonstrated. Then, Antoine looked at Gdt1p regulation. He observed that Gdt1p is rapidly downregulated as a response to extracellular Mn2+ addition and that regulation occurs both at transcriptional and post-translational levels.