 Aims
This course will illustrate the principles of the basic techniques used in practical biochemistry and will provide to the students the opportunity to perform experiments in practical courses.
Main themes
The course will illustrate the basic and most widely used biochemical techniques allowing DNA and protein purification, identification and their biochemical properties. Practical courses are organized to illustrate these techniques.
Content and teaching methods
Chapters :
1) Centrifugation and subcellular fractionation
2) Chromatography
3) 1D and 2D electrophoresis
4) Membrane protein purification
5) Microscopic techniques
6) Protein identification by Edman sequencing and mass spectrometry
7) Immunological techniques
8) DNA sequencing
9) Genotype identification based on DNA probes and PCR techniques
Other information (prerequisite, evaluation (assessment methods), course materials recommended readings, ...)
Basic Biochemistry course is required
Other credits in programs
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BIR22/0C
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Deuxième année du programme conduisant au grade de bio-ingénieur: Chimie et bio-industries (Technologies & gestion de l'information)
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(3 credits)
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Mandatory
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BIR22/1C
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Deuxième année du programme conduisant au grade de bio-ingénieur: Chimie et bio-industries (Sciences, technologie & qualité des aliments)
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(3 credits)
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Mandatory
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BIR22/2C
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Deuxième année du programme conduisant au grade de bio-ingénieur : Chimie et bio-industries (Ingénierie biomoléculaire et cellulaire)
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(3 credits)
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Mandatory
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BIR22/3C
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Deuxième année du programme conduisant au grade de bio-ingénieur : Chimie et bioindustries (Nanobiotechnologies, matériaux et catalyse)
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(3 credits)
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Mandatory
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BIR22/4C
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Deuxième année du programme conduisant au grade de bio-ingénieur : Chimie et bio-industries (Technologies environnementales: eau, sol, air)
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(3 credits)
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Mandatory
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