3.00 credits
30.0 h
Q1
Teacher(s)
Bertrand Luc (coordinator); Dumoutier Laure; Laloux Géraldine; Limaye Nisha;
Language
French
Prerequisites
The prerequisite(s) for this Teaching Unit (Unité d’enseignement – UE) for the programmes/courses that offer this Teaching Unit are specified at the end of this sheet.
Main themes
Tools for cloning and expression in prokaryotes and eukaryotes. Genetic engineering for the production of proteins useful for biomedical and pharmaceutical sciences. Study of genetic variants for diagnostic purposes and responsible for genetic diseases. Gene and cell therapies.
Content
Four main topics will be proposed to masters students in biomedical sciences (SBIM Master) : 1) Molecular biology of prokaryotes (plasmid, cloning, bacterial expression of proteins, site-directed mutagenesis); 2) Molecular biology of eukaryotes I (DNA Structure, analysis of variants, sequencing, quantitative PCR, RNAseq); 3) Molecular biology of eukaryotes II (cloning, vectors and protein expression, transfection and viral infection, µRNA, RNA interference, transgenesis); 4) Gene and cellular therapies (CRISPR/cas9, IPS cells, engineered tissues)
Evaluation methods
Written exam including questions on each of the 4 topics.
Language : French with specific part in English
Language : French with specific part in English
Other information
PDF versions of slides presented in the course, which cover the subject in a comprehensive way, will be made available on MoodleUCL. There is no formal syllabus.
Faculty or entity
SBIM
