3.00 credits
20.0 h + 30.0 h
Q2
Teacher(s)
Bertrand Luc;
Language
French
Prerequisites
The prerequisite(s) for this Teaching Unit (Unité d’enseignement – UE) for the programmes/courses that offer this Teaching Unit are specified at the end of this sheet.
Main themes
The basic experimental techniques commonly used in molecular biology (enzymatic digestion, cDNA cloning, PCR, gel electrophoresis and bacteria transformation) and in biochemistry (protein purification and assay, western blot, enzyme activity measurements).
Learning outcomes
At the end of this learning unit, the student is able to : | |
| 1 | - Correctly use a pipet, present results clearly and interpret them. - Keep a lab book updated. - Use basic approaches in molecular biology and biochemistry encountered daily in biomedical sciences. |
Content
Student will practice with molecular andd biochemical tools relative to the enzyme called phosphoserine phosphatase. The different steps will be the cloning of its cDNA in a bacterial vector to promote its overexpression, its purification and the measurement of its activity.
Teaching methods
Practical courses with active participation of the student.
Evaluation methods
The global evalaution will take into account different parameters, including :
- The general behavior during practical course.
- Daily evaluations
- A report consisting of the lab book
- The general behavior during practical course.
- Daily evaluations
- A report consisting of the lab book
Faculty or entity
FASB
