Note from June 29, 2020
Although we do not yet know how long the social distancing related to the Covid-19 pandemic will last, and regardless of the changes that had to be made in the evaluation of the June 2020 session in relation to what is provided for in this learning unit description, new learnig unit evaluation methods may still be adopted by the teachers; details of these methods have been - or will be - communicated to the students by the teachers, as soon as possible.
Although we do not yet know how long the social distancing related to the Covid-19 pandemic will last, and regardless of the changes that had to be made in the evaluation of the June 2020 session in relation to what is provided for in this learning unit description, new learnig unit evaluation methods may still be adopted by the teachers; details of these methods have been - or will be - communicated to the students by the teachers, as soon as possible.
4 credits
30.0 h
Q1
Teacher(s)
Bertrand Luc; Collet Jean-François; Gatto Laurent; Laloux Géraldine; Rider Mark (coordinator);
Language
English
Prerequisites
Good background in chemistry, physics and biochemistry. Basic computer skills (including use of search engines and data base searching).
Main themes
Methods of expression, purification an in vitro renaturation of proteins (5h)
Protein sequencing (2h)
Bioinformatic analysis of proteins (homology searches, alignments, phylogenetic studies, motif and domain searching, structure modelling) (10h)
Structure determination by NMR, crystal structures by X-ray diffraction in relation to function (3h)
Enzymology (thermodynamics, pre- and steady state kinetics, calculation of kinetic parameters, ligand binding and allosteric enzymes, site-directed mutagenesis, theory of metabolic control) (10 h)
Protein sequencing (2h)
Bioinformatic analysis of proteins (homology searches, alignments, phylogenetic studies, motif and domain searching, structure modelling) (10h)
Structure determination by NMR, crystal structures by X-ray diffraction in relation to function (3h)
Enzymology (thermodynamics, pre- and steady state kinetics, calculation of kinetic parameters, ligand binding and allosteric enzymes, site-directed mutagenesis, theory of metabolic control) (10 h)
Aims
At the end of this learning unit, the student is able to : | |
| 1 |
To provide Masters students in Biomedical Sciences with the necessary competence to study: - techniques of overexpression/purification and structural analysis of proteins - structure-function relationships in proteins - physiological roles of enzymes |
The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.
Content
The aim is to teach to masters students in biomedical sciences (SBIM Master) techniques of enzymology and metabolic control analysis, purification, sequencing, structure determination and bioinformatic analysis of proteins.
Content:
Expression, purification and renaturation of proteins in vitro (6h)
Protein sequencing (3h)
Bioinformatic analysis of proteins (3h)
Techniques for determining the 3D structures of proteins (3h)
Analysis of protein-protein interactions (3h)
Kinetics and mechanisms of enzymes, cooperativity, structure-fuction relationships, epresentation of protein structures, metabolic control analysis (12h)
Content:
Expression, purification and renaturation of proteins in vitro (6h)
Protein sequencing (3h)
Bioinformatic analysis of proteins (3h)
Techniques for determining the 3D structures of proteins (3h)
Analysis of protein-protein interactions (3h)
Kinetics and mechanisms of enzymes, cooperativity, structure-fuction relationships, epresentation of protein structures, metabolic control analysis (12h)
Teaching methods
Formal lectures plus a "workshop" on bioinformatic analysis.
Evaluation methods
Written exam of 3 h duration and 6 questions with at least one question from each lecturer. The exam questions will be in English but the students are free to answer either in French or in English.
There are no negative points or ponderation according to the questions and course content. However, when a student has a mark between 9/20 et 10/20 after correction, the lecturers re-examine all the points to decide if the mark should be rounded up to 10 or stay at 9. If the answers are insufficient, the final mark will be rounded down.
There are no negative points or ponderation according to the questions and course content. However, when a student has a mark between 9/20 et 10/20 after correction, the lecturers re-examine all the points to decide if the mark should be rounded up to 10 or stay at 9. If the answers are insufficient, the final mark will be rounded down.
Other information
Teaching of the course material will be in English and the Power Point files will be mostly in English.
Online resources
There is no formal syllabus ! PDF versions of slides presented in the course, which cover the subject in a comprehensive way, will be made available on MoodleUCL (https://moodleucl.uclouvain.be/).
In addition, a tablet will be used to explain certain aspects of the course. The "Tablet" PDF versions of the PowerPoint files will also be made available to students via MoodleUCL.
Bibliography
Les étudiants sont encouragés à lire des revues dans la littérature !
The students are encouraged to do background reading, for example by consulting numerous comprehensive reviews on the subject matter of the course available in the literature (for example via Pub Med).
The students are encouraged to do background reading, for example by consulting numerous comprehensive reviews on the subject matter of the course available in the literature (for example via Pub Med).
Faculty or entity
SBIM
