4 credits
30.0 h
Q1
Teacher(s)
Bertrand Luc; Collet Jean-François; De Plaen Etienne; Rider Mark coordinator;
Language
English
Prerequisites
Good background in chemistry, physics and biochemistry. Basic computer skills (including use of search engines and data base searching).
Main themes
Methods of expression, purification an in vitro renaturation of proteins (5h)
Protein sequencing (2h)
Bioinformatic analysis of proteins (homology searches, alignments, phylogenetic studies, motif and domain searching, structure modelling) (10h)
Structure determination by NMR, crystal structures by X-ray diffraction in relation to function (3h)
Enzymology (thermodynamics, pre- and steady state kinetics, calculation of kinetic parameters, ligand binding and allosteric enzymes, site-directed mutagenesis, theory of metabolic control) (10 h)
Protein sequencing (2h)
Bioinformatic analysis of proteins (homology searches, alignments, phylogenetic studies, motif and domain searching, structure modelling) (10h)
Structure determination by NMR, crystal structures by X-ray diffraction in relation to function (3h)
Enzymology (thermodynamics, pre- and steady state kinetics, calculation of kinetic parameters, ligand binding and allosteric enzymes, site-directed mutagenesis, theory of metabolic control) (10 h)
Aims
At the end of this learning unit, the student is able to : | |
| 1 | To provide Masters students in Biomedical Sciences with the necessary competence to study: - techniques of overexpression/purification and structural analysis of proteins - structure-function relationships in proteins - physiological roles of enzymes |
The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.
Content
Enzymology, purification, sequencing, structure determination and bioinformatic analysis of proteins.
Teaching methods
Formal lectures plus a "workshop" on bioinformatic analysis.
Evaluation methods
Written exam
Other information
Teaching of the course material will be in English and the Power Point files will be mostly in English.
Bibliography
- fichier PDF des présentation powerpoint du cours disponibles sur moodle
The students are encouraged to do background reading, for example by consulting numerous comprehensive reviews on the subject matter of the course available in the literature (for example via Pub Med).
Teaching materials
- fichier PDF des présentation powerpoint du cours disponibles sur moodle
Faculty or entity
SBIM
