2.0 credits
30.0 h
1q
Teacher(s)
De Plaen Etienne ;
Octave Jean-Noël (coordinator) ;
Language
Français
Main themes
Cloning and expression in prokaryotic and eukaryotic cells. Genetic engineering and production of proteins that are useful in biomedical and pharmaceutical sciences. Utilization of DNA polymorphisms as diagnostic tools. Transgenic animals. DNA modifications in hereditary diseases. Gene therapy.
Aims
The objectives are to learn the approaches of molecular biology, which allow the analysis of DNA and RNA to develop both diagnostic tools as well as cellular and animal models needed in biomedical and pharmaceutical sciences.
The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.
Content
Description and utilization of different cloning and expression vectors in procaryotic cells. Construction and screening of genomic DNA libraries. Analysis of the DNA molecule : Detection of different polymorphisms. Construction and screening of cDNA libraries. Cloning and expression of cDNA in different cell types. Overexpression and knock out of genes in transgenic animals. Examples of gene therapy.
Other information
Assessment: By written exam.
Support: Notes and Power Point presentations provided by the lecturers.
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