{"id":8403,"date":"2020-07-27T12:23:19","date_gmt":"2020-07-27T10:23:19","guid":{"rendered":"https:\/\/sites.uclouvain.be\/soumillionlab\/?p=8403"},"modified":"2020-07-28T10:30:34","modified_gmt":"2020-07-28T08:30:34","slug":"chromosomal-libraries","status":"publish","type":"post","link":"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/","title":{"rendered":"Chromosomal Libraries Chapter is out!"},"content":{"rendered":"\n

A lot of the work we do in the laboratory is trying to find better ways to do Directed Evolution. One of the thing we believe is that expressing libraries in their native genomic context let you navigate the fitness landscape better.

If you want to find out how exactly we build those libraries, the detailed protocols we use, both in E. coli<\/em> and in S. thermophilus<\/em> have now been published as a book chapter<\/a> by Springer Protocols.

“Dorrazehi G.M., Worms S., Chirakadavil J.B., Mignolet J., Hols P., Soumillion P. (2020) Building Scarless Gene Libraries in the Chromosome of Bacteria. In: Iranzo O., Roque A. (eds) Peptide and Protein Engineering. Springer Protocols Handbooks. Humana, New York, NY

Open access:
https:\/\/dial.uclouvain.be\/pr\/boreal\/object\/boreal:231618<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

A lot of the work we do in the laboratory is trying to find better ways to do Directed Evolution. One of the thing we believe is that expressing libraries in their native genomic context let you navigate the fitness<\/p>\n","protected":false},"author":73,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[13],"tags":[],"class_list":["post-8403","post","type-post","status-publish","format-standard","hentry","category-news"],"yoast_head":"\nChromosomal Libraries Chapter is out! : Soumillion Laboratory<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Chromosomal Libraries Chapter is out! : Soumillion Laboratory\" \/>\n<meta property=\"og:description\" content=\"A lot of the work we do in the laboratory is trying to find better ways to do Directed Evolution. One of the thing we believe is that expressing libraries in their native genomic context let you navigate the fitness\" \/>\n<meta property=\"og:url\" content=\"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/\" \/>\n<meta property=\"og:site_name\" content=\"Soumillion Laboratory\" \/>\n<meta property=\"article:published_time\" content=\"2020-07-27T10:23:19+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-07-28T08:30:34+00:00\" \/>\n<meta name=\"author\" content=\"Gol Dorrazehi\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Gol Dorrazehi\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/2020\\\/07\\\/27\\\/chromosomal-libraries\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/2020\\\/07\\\/27\\\/chromosomal-libraries\\\/\"},\"author\":{\"name\":\"Gol Dorrazehi\",\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/#\\\/schema\\\/person\\\/5b487cc1fa8be3bdb4013798ae49b9a3\"},\"headline\":\"Chromosomal Libraries Chapter is out!\",\"datePublished\":\"2020-07-27T10:23:19+00:00\",\"dateModified\":\"2020-07-28T08:30:34+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/2020\\\/07\\\/27\\\/chromosomal-libraries\\\/\"},\"wordCount\":133,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/#organization\"},\"articleSection\":[\"News\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/2020\\\/07\\\/27\\\/chromosomal-libraries\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/2020\\\/07\\\/27\\\/chromosomal-libraries\\\/\",\"url\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/2020\\\/07\\\/27\\\/chromosomal-libraries\\\/\",\"name\":\"Chromosomal Libraries Chapter is out! : Soumillion Laboratory\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/#website\"},\"datePublished\":\"2020-07-27T10:23:19+00:00\",\"dateModified\":\"2020-07-28T08:30:34+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/2020\\\/07\\\/27\\\/chromosomal-libraries\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/2020\\\/07\\\/27\\\/chromosomal-libraries\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/2020\\\/07\\\/27\\\/chromosomal-libraries\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Chromosomal Libraries Chapter is out!\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/#website\",\"url\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/\",\"name\":\"Soumillion Laboratory\",\"description\":\"Evolution. Directed.\",\"publisher\":{\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/#organization\",\"name\":\"Soumillion Laboratory\",\"url\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/wp-content\\\/uploads\\\/2018\\\/10\\\/SL1@300x.png\",\"contentUrl\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/wp-content\\\/uploads\\\/2018\\\/10\\\/SL1@300x.png\",\"width\":1555,\"height\":939,\"caption\":\"Soumillion Laboratory\"},\"image\":{\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/#\\\/schema\\\/logo\\\/image\\\/\"}},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/#\\\/schema\\\/person\\\/5b487cc1fa8be3bdb4013798ae49b9a3\",\"name\":\"Gol Dorrazehi\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/181ae1fd1beac3be80d50e27ebbfedb2c8e491965b4d1e4c9277694fe6583aaf?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/181ae1fd1beac3be80d50e27ebbfedb2c8e491965b4d1e4c9277694fe6583aaf?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/181ae1fd1beac3be80d50e27ebbfedb2c8e491965b4d1e4c9277694fe6583aaf?s=96&d=mm&r=g\",\"caption\":\"Gol Dorrazehi\"},\"url\":\"https:\\\/\\\/sites.uclouvain.be\\\/soumillionlab\\\/author\\\/gol\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Chromosomal Libraries Chapter is out! : Soumillion Laboratory","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/","og_locale":"en_US","og_type":"article","og_title":"Chromosomal Libraries Chapter is out! : Soumillion Laboratory","og_description":"A lot of the work we do in the laboratory is trying to find better ways to do Directed Evolution. One of the thing we believe is that expressing libraries in their native genomic context let you navigate the fitness","og_url":"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/","og_site_name":"Soumillion Laboratory","article_published_time":"2020-07-27T10:23:19+00:00","article_modified_time":"2020-07-28T08:30:34+00:00","author":"Gol Dorrazehi","twitter_card":"summary_large_image","twitter_misc":{"Written by":"Gol Dorrazehi","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/#article","isPartOf":{"@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/"},"author":{"name":"Gol Dorrazehi","@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/#\/schema\/person\/5b487cc1fa8be3bdb4013798ae49b9a3"},"headline":"Chromosomal Libraries Chapter is out!","datePublished":"2020-07-27T10:23:19+00:00","dateModified":"2020-07-28T08:30:34+00:00","mainEntityOfPage":{"@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/"},"wordCount":133,"commentCount":0,"publisher":{"@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/#organization"},"articleSection":["News"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/","url":"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/","name":"Chromosomal Libraries Chapter is out! : Soumillion Laboratory","isPartOf":{"@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/#website"},"datePublished":"2020-07-27T10:23:19+00:00","dateModified":"2020-07-28T08:30:34+00:00","breadcrumb":{"@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/2020\/07\/27\/chromosomal-libraries\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/sites.uclouvain.be\/soumillionlab\/"},{"@type":"ListItem","position":2,"name":"Chromosomal Libraries Chapter is out!"}]},{"@type":"WebSite","@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/#website","url":"https:\/\/sites.uclouvain.be\/soumillionlab\/","name":"Soumillion Laboratory","description":"Evolution. Directed.","publisher":{"@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/sites.uclouvain.be\/soumillionlab\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/#organization","name":"Soumillion Laboratory","url":"https:\/\/sites.uclouvain.be\/soumillionlab\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/#\/schema\/logo\/image\/","url":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-content\/uploads\/2018\/10\/SL1@300x.png","contentUrl":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-content\/uploads\/2018\/10\/SL1@300x.png","width":1555,"height":939,"caption":"Soumillion Laboratory"},"image":{"@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/sites.uclouvain.be\/soumillionlab\/#\/schema\/person\/5b487cc1fa8be3bdb4013798ae49b9a3","name":"Gol Dorrazehi","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/181ae1fd1beac3be80d50e27ebbfedb2c8e491965b4d1e4c9277694fe6583aaf?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/181ae1fd1beac3be80d50e27ebbfedb2c8e491965b4d1e4c9277694fe6583aaf?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/181ae1fd1beac3be80d50e27ebbfedb2c8e491965b4d1e4c9277694fe6583aaf?s=96&d=mm&r=g","caption":"Gol Dorrazehi"},"url":"https:\/\/sites.uclouvain.be\/soumillionlab\/author\/gol\/"}]}},"_links":{"self":[{"href":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-json\/wp\/v2\/posts\/8403","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-json\/wp\/v2\/users\/73"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-json\/wp\/v2\/comments?post=8403"}],"version-history":[{"count":4,"href":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-json\/wp\/v2\/posts\/8403\/revisions"}],"predecessor-version":[{"id":8653,"href":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-json\/wp\/v2\/posts\/8403\/revisions\/8653"}],"wp:attachment":[{"href":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-json\/wp\/v2\/media?parent=8403"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-json\/wp\/v2\/categories?post=8403"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.uclouvain.be\/soumillionlab\/wp-json\/wp\/v2\/tags?post=8403"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}