Basic technical services in clinical biology: routine and on-call

Teacher(s)

Mullier François; Saussoy Pascale (coordinator); van Dievoet Marie-Astrid;

Language

French

Main themes

Morphology (5 hours) : - Automated counting of the blood cells - Errors in an Haematological Morphology Lab - Quality control (including the evaluation of an automated haematology counter) - Microscopy : principles, special optical systems, electron microscopy - General morphological methods : harvesting, fixation, differentiation,… - Special morphological methods : cytochemistry, cytoenzymology, immunocytochemistry, autoradiography Immuno-haematology (5 hours) : - Blood groups - Irregular anti-erythrocyte allo-antibodies - Anti-erythrocyte auto-antibodies - Pretransfusion cross-matching - Flow cytometry : principles and applications Transfusion (4 hours) : - Blood donation - Organization in a blood transfusion centre - Red cell transfusion - Labile and stable blood components - Transfusion complications Haemostasis (6 hours) : - Routine tests in coagulation - Specific quantitative analysis of factor VIII, von Willebrand, IX, XI, XII, and prenatal diagnosis of haemophilia and von Willebrand disease - Specific quantitative analysis of factor I, II, V, VII, X - Dysfibrinogenemia evaluation - Thrombophilic evaluation - Activation markers in haemostasis - Screening and quantitative analysis of natural and pathological coagulation inhibitors - Control of the efficacy of anticoagulant and anti-aggregation treatment - Evaluation of the fibrinolysis and control of thrombolytic treatment

Learning outcomes

At the end of this learning unit, the student is able to :
1	Explanation of the methods used in morphology, immuno-haematology, blood transfusion and haemostasis. Teaching interpretation of the results in relationship with the clinical situations. Quality control and general organization in a Haematology Lab.

Content

Practical  and teaching  about the medical supervision of the emergency tests (haemostasis, blood transfusion and morphology)

Teaching methods

Practical (training in the field by routine teams) and theoretical

Evaluation methods

Various skills must be acquired at the end of the training in the 3 sectors (chemistry, hematology and microbiology)

Online resources

NA

Faculty or entity

MED