2.00 credits
30.0 h
Q2
Teacher(s)
Dauguet Nicolas; Dumoutier Laure (coordinator);
Language
French
Prerequisites
The prerequisite(s) for this Teaching Unit (Unité d’enseignement – UE) for the programmes/courses that offer this Teaching Unit are specified at the end of this sheet.
Main themes
-culture of cell lines in sterile conditions ;
-cell analysis by fluorescent microscopy ;
-biochemical assays (proteins, cell proliferation and cell survival assays) ;
-introduction to FACS analysis and study of the characteristic profile of selected cell populations.
Learning outcomes
At the end of this learning unit, the student is able to : | |
| 1 | The aim is to learn the basic techniques of cell biology: -cell culture and propagation ; -morphological and microscopic examination of the cells ; -analysis of cell proliferation and cell survival, -introduction to the analysis of cell populations by FACS (Fuorescence-Associated Cell Sorter). Our aim is also to train students to write a laboratory notebook and a training course report. |
Content
This training course takes place during 5 consecutive afternoons in two research laboratories of the Faculty. Students will work in small groups (usually 2 students) under the supervision of a research scientist.
Teaching methods
Training course and lecture on the flow cytometer.
Supervision : three assistants and the professor.
Supervision : three assistants and the professor.
Evaluation methods
Continuous assessment and writing of a training course report.
Written exam.
Failure to submit training course report will lead to a global note of 0.
Written exam.
Failure to submit training course report will lead to a global note of 0.
Online resources
Course notes and slides available on Moodle.
Faculty or entity
SBIM
