Introduction to protein biotechnology

lchm2170  2022-2023  Louvain-la-Neuve

Introduction to protein biotechnology
3.00 credits
22.5 h + 7.5 h
Q1
Teacher(s)
Morsomme Pierre; Morsomme Pierre (compensates Soumillion Patrice); Soumillion Patrice;
Language
Main themes
1. Gene manipulation: recovery, cloning, modification, transfer and characterisation.
2. Gene expression: vectors, expression in bacteria, yeasts, plants, insect and mammalian cells, production of monoclonal antibodies.
3. Protein improvement: genetic engineering, directed evolution and chemical stabilisation.
All the underlying techniques will be briefly explained.
Learning outcomes

At the end of this learning unit, the student is able to :

1 The student will get to know the field of protein biotechnology for which the interactions with chemistry are continuously growing, especially in bio-pharmacy. He will learn the notions of molecular biology and genetic engineering that are useful with regard to the production and improvement of proteins.
Another objective is also to acquire the vocabulary associated with these notions so that the student will later be able to interact with the experts of that field.
 
Content
1. Gene manipulation: recovery, cloning, modification, transfer and characterisation.
2. Gene expression: vectors, expression in bacteria, yeasts, plants, insect and mammalian cells, production of monoclonal antibodies.
3. Protein improvement: genetic engineering, directed evolution and chemical stabilisation. All the underlying techniques will be briefly explained.
Teaching methods
Ex cathedra lectures
Evaluation methods
Written exam
Online resources
All documents are proposed via Moodle
Teaching materials
  • matériel sur moodle
Faculty or entity
CHIM


Programmes / formations proposant cette unité d'enseignement (UE)

Title of the programme
Sigle
Credits
Prerequisites
Learning outcomes
Master [120] in Chemical and Materials Engineering

Master [120] in Chemistry

Master [60] in Chemistry