Teacher(s)

Morsomme Pierre;

Language

French

Main themes

This course covers classic methods used to purify biological macromolecules et determine their identity and biochemical properties. Practicals illustrate standard techniques used in analytical biochemistry.

Content

Centrifugation and fractionation of cells, organelles or molecules. Protein chromatography techniques. Protein electrophoresis (1D and 2D). Light and fluorescence microscopy of proteins. Mass spectrometry analysis and sequencing of proteins. Immunodetection (ELISA, western blotting, in situ).

Teaching methods

Due to the COVID-19 crisis, the information in this section is particularly likely to change.

The theoretical part will be taught by the teacher using the blackboard and Power Point files.
Practicals will give the students (groups of two) the opportunity to put in practice the methodologies taught in the theoretical part.

Evaluation methods

Due to the COVID-19 crisis, the information in this section is particularly likely to change.

An exam will be performed at the end of the practicals to assess the comprehension of the methodologies used (25% of the final score).
An exam on the theoretical part will be organized to assess the understanding of the various concepts as well as the capacity to use these concepts to solve practical problems (75% of the final score).

Other information

Participation in the practical work is mandatory. Any unjustified absence will result in a penalty on the final grade of the course.
Ths course can be given in english.

Online resources

Moodle

Bibliography

Synthèse

Faculty or entity

AGRO