Note from June 29, 2020
Although we do not yet know how long the social distancing related to the Covid-19 pandemic will last, and regardless of the changes that had to be made in the evaluation of the June 2020 session in relation to what is provided for in this learning unit description, new learnig unit evaluation methods may still be adopted by the teachers; details of these methods have been - or will be - communicated to the students by the teachers, as soon as possible.
Although we do not yet know how long the social distancing related to the Covid-19 pandemic will last, and regardless of the changes that had to be made in the evaluation of the June 2020 session in relation to what is provided for in this learning unit description, new learnig unit evaluation methods may still be adopted by the teachers; details of these methods have been - or will be - communicated to the students by the teachers, as soon as possible.
4 credits
36.0 h + 6.0 h
Q1
Teacher(s)
Morsomme Pierre; Soumillion Patrice;
Language
French
Main themes
The course will be divided into two parts:1. Structural biochemistry:- patterns and forms in protein structures (observation, manipulation, description, classification)- principles of protein folding - domains and assemblies (modular nature of proteins, multi-protein complexes)- bioinformatics in structural biology- interactions between biomolecules (methods and characterization)2. Enzymology- principles of enzyme catalysis (Michaelis Menten, steady state kinetics, reaction schemes)- the basics of chemical catalysis by proteins (catalysis by amino acids lateral chains, active site complementarity, entropic catalysis, transition state stabilisation)- control of enzyme activity (inhibition, activation, cooperativity, allosterism, environmental effects)
Aims
At the end of this learning unit, the student is able to : | |
| 1 | The objective of the course is to acquire the fundamental principles that allow describing and understanding the properties of proteins and nucleic acids, going from structural biology to chemistry (catalysis, interactions). It will give a vision of the general characteristics of proteins and nucleic acids architecture that lead to the very great variety of structures, functions and interactions observed in nature. The student will also understand the chemical nature of molecular interactions and the mechanisms of chemical catalysis that underlie the functions of proteins. Regarding structural biochemistry, the student will acquire the knowledge of the concepts and tools that are necessary for manipulating, observing and describing the three-dimensional structures of biomolecules. The student will learn how to use the variety of informatic resources related to structural biology and available on the web (databanks, prediction algorithms, visualization softwares). He will also learn how to identify, characterize and describe interactions between biomolecules.The formalism of enzymatic kinetics will be initially reminded. With the help of case studies, the student will learn how to identify the different catalytic strategies used by enzymes and the underlying principles of chemical catalysis. These notions will finally allow him/her to better understand the different molecular mechanisms of enzymatic regulation and inhibition. |
The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.
Content
About 24 hours will be dedicated to lectures with the help of PowerPoint slides. 6 hours will be organized in computer room for exercises or guided tours of various websites.
Content:
1. Amino acids side chains:
- hydrophobicity/hydrophilicity
- electrostatic and acido-basic properties
- nucleophilicity
- redox properties
2. Structural biochemistry:
- biomolecule structures and interactions: non covalent driving forces and quantitative aspects
- thermodynamical and chemical stability of proteins
- protein folding : from molecular mechanisms to conformational diseases
- observation, manipulation, visualisation, description and classification of three-dimensional structures (in computer room)
3. Enzymology
- principles of enzyme catalysis (Michaelis Menten, steady state kinetics, reaction schemes)
- the basics of chemical catalysis by proteins (catalysis by amino acids lateral chains, active site complementarity, entropic catalysis, transition state stabilisation)
- cofactors and coenzymes chemistries
- control of enzyme activity (inhibition, activation, cooperativity, allosterism, environmental effects)
- numerical simulation of enzymatic catalysis (in computer room)
Teaching methods
Ex cathedra lectures
Evaluation methods
Written exam
Other information
Precursory courses: Basics in biochemistry (e.g. Elements of biochemistry CHM1271)
Online resources
All documents are available via Moodle
Teaching materials
- transparents sur moodle
Faculty or entity
BIOL
