3 credits
30.0 h
Q1
Teacher(s)
Alsteens David; Hachez Charles coordinator; Hols Pascal;
Language
French
Prerequisites
General microbiology and general biochemistry
Biological and microbial engineering
Biological and microbial engineering
Main themes
The different types of microbial, animal and plant cell cultures will be presented. The importance of the culture medium composition and of the culture parameters will be described. Problems related to scaling-up will be highlighted. Several examples of biomedical and industrial applications will be illustrated.
Aims
At the end of this learning unit, the student is able to : | |
| 1 | a. Contribution de l'activité au référentiel AA (AA du programme) Cohérence des AA cours en regard de ceux du programme 1.2 2.2 3.1 6.1, 6.2, 6.4
b. Formulation spécifique pour cette activité des AA du programme By the end of this course, the student should be able: - To explain the methods used for growing microbial, animal and plant cells - To explain the major applications of cell cultures - To understand and set out examples of cell cultures in the microbial, animal and plant fields as described in English scientific journals |
The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.
Content
Microbial cells: culture methods, influence of the medium composition and physico-chemical parameters, combining of metabolic engineering and culture parameters, examples of production of metabolites
Animal cells (mammals and insects): general properties, adhesive and non-adhesive cells, culture medium composition, serum-free medium, physical parameters, examples of protein production, hybridoma and monoclonal antibody production
Plant cells: cell, tissue and organ culture, culture medium composition, bioreactor design, example of production of pharmacological metabolites and proteins.
Animal cells (mammals and insects): general properties, adhesive and non-adhesive cells, culture medium composition, serum-free medium, physical parameters, examples of protein production, hybridoma and monoclonal antibody production
Plant cells: cell, tissue and organ culture, culture medium composition, bioreactor design, example of production of pharmacological metabolites and proteins.
Teaching methods
The theoretical part will be presented by the teacher using the blackboard and PowerPoint files.
Students will illustrate applications of cell cultures by presenting a paper from the scientific literature
Students will illustrate applications of cell cultures by presenting a paper from the scientific literature
Evaluation methods
Assessment will be based on the presentation of a paper from the English scientific literature illustrating an application of cell culture in the microbial, animal or plant field. Emphasis will put on the following aspects: ability to analyze and synthesize the data, didactic quality of the presentation and the PowerPoint support, capacity to address questions from the audience
Other information
This course can be given in English.
Online resources
Moodle
Bibliography
ouvrages de référence mentionnés au premier cours (achat non-obligatoire)
Faculty or entity
AGRO
Programmes / formations proposant cette unité d'enseignement (UE)
Title of the programme
Sigle
Credits
Prerequisites
Aims
Master [120] in Agricultural Bioengineering
Master [120] in Chemistry and Bioindustries
Master [120] in Biochemistry and Molecular and Cell Biology
Master [120] in Biomedical Engineering
Master [60] in Biology