Genetic engineering

lbrmc2101  2018-2019  Louvain-la-Neuve

Genetic engineering
3 credits
30.0 h + 7.5 h
Q1
Teacher(s)
Chaumont François coordinator; Hachez Charles;
Language
French
Prerequisites
General biochemistry and general genetics

The prerequisite(s) for this Teaching Unit (Unité d’enseignement – UE) for the programmes/courses that offer this Teaching Unit are specified at the end of this sheet.
Main themes
The theoretical part will detail the major steps of genetic engineering: preparation and screening of libraries, gene cloning, gene characterization and modification, gene expression in heterologous hosts. Concrete problems of genetic engineering in the microbial, animal and plant fields will be discussed. Recent examples of genetic engineering achievements from the recent literature will be discussed.
Aims

At the end of this learning unit, the student is able to :

1

a. Contribution de l'activité au référentiel AA (AA du programme)

Cohérence des AA cours en regard de ceux du programme

1.2, 1.3

2.2

3.4, 3.9

6.1, 6.2

 

b. Formulation spécifique pour cette activité des AA du programme

By the end of this course, the student should be able:

-        To explain the main genetic engineering methodologies

-        To choose, according to the problem posed, among different strategies used to clone a gene, modify it and transfer it into other organisms

-        To propose experimental approaches aimed at solving practical problems of genetic engineering in the microbial, animal and plant fields

-        To understand and set out examples of genetic engineering in the microbial, animal and plant fields as described in English scientific journals

 

The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.
Content
Theoretical part: Methods of genomic and cDNA screening - Global analysis of the genome and its expression (genomics, transcriptomics, proteomics, metabolomics) - directed mutagenesis - gene expression in heterologous hosts: Escherichia coli, other bacteria, yeast, transgenic cell lines and transgenic organisms (animals and plants) - protein engineering - genic therapy.
Solving problems: concrete problems of genetic engineering will be exposed and solved by the students.
Teaching methods
The course is organized as a flipped classroom. Students are divided into working groups during the first course and a theme is assigned to each group. They will develop the theoretical aspects of a model organism used in genetic engineering and will answer a thematic question asked by the teachers.
In addition, classroom exercises are organised. Concrete problems of genetic engineering are submitted to the students who will propose solutions that will be discussed all together.
Evaluation methods
Assessment is based on, on the one hand, the preparation and presentation of the theme to the class and, on the other hand, an open-book written examination on the solving of problems of genetic engineering.
Students are required to self-assess themselves on flipped classroom work and this self-assessment can be used to adapt the grades.
Other information
This course can be given in English.
Participation in the first course is mandatory for the organization of the flipped classroom.
Online resources
Moodle
Bibliography
Syllabus et ouvrages de référence mentionnés au premier cours
Faculty or entity
AGRO


Programmes / formations proposant cette unité d'enseignement (UE)

Title of the programme
Sigle
Credits
Prerequisites
Aims
Master [120] in Chemistry and Bioindustries

Master [120] in Biochemistry and Molecular and Cell Biology

Master [120] in Biomedical Engineering

Master [60] in Biology