At the end of this learning unit, the student is able to : | |
| 1 | a. Contribution of the activity to the AA referencial (AA of the programme) The course contributes to develop and evaluate the learning outcomes listed below from the BIRC21 master programme: 1.1., 1.3, 2.3, 3.4, 3.5, 3.6, 3.7, 3.8, 6.1, 6.2, 6.5, 7.1
b. Specific formulation for this activity of AA from the programme (maximum 10) At the end of this activity, the student will be able to: - describe active principles for spectroscopic and electrochemical analyses methods as well as experimental aspects, limitations and related performances; - discuss the medium effect on acid-base reactions; - apply a professional practice to chemistry laboratory for current analyses methods: critics and adaptation of analytical protocols, performances (influence of methods, instrumentation and operators) ; - correlate theory to experiment; - structure and synthetize the information gathered in lab reports of different kind (brief or full reports) ; - apply statistical tools for data treatment ; - propose a reliable work plan to solve an analytical chemistry problem. At the end of the class, the student will have developed personal skills which comprises: laboratory good practices, work planning, team work, creativity and initiative towards practical processes |
The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.
Electrolysis-based analyses methods: analytical applications, voltamperometry. Molecular absorption spectroscopy: dispersive instruments and FT-IR spectrometers, tuning, performances, applications.
Atomic spectroscopy: overview, apparatuses, performances, matrix effects.
Acid-base reactions in non-aqueous media: solvent types and pH calculations.
Application of X-ray diffraction and fluorescence in analytical chemistry.
B. Exercises and demonstrations
- Data analysis from electrochemical methods
- Demonstrations : atomic absorption spectroscopy, X-ray diffraction
C. Laboratory work
a) Development of a measuring protocol for the determination of the enzyme activity that includes the use of colorimetry, the solubilization of the enzyme and the incubation protocol. Method: Realization of two measuring cycles; brain storming between two student's pairs.
b) Titration in non-aqueous media
c) Proteins titration ' comparison of performances from two different methods based on UV-visible spectroscopy.
This course can be given in English.
syllabus pour le cours et fascicule pour les exercices pratiques
Livre de référence : Fundamentals of Analytical Chemistry, D. A. Skoog, D. M. West, F. J. Holler, S. R. Crouch, 8th ed., Thomson Brooks/Cole, 2004.
- support de cours facultatif : Quantitative Chemical Analysis, D. C. Harris, 8th ed., W. H. Freeman & Co., 2010
