Organic analysis I : separation techniques

6 credits

60.0 h + 30.0 h

Teacher(s)

Collin Sonia;

Language

French

Prerequisites

Organic chemistry CHIM1170

Main themes

- Properties of chemical compounds
- Strategy of an organic analysis
- Methods for extraction and concentration
- Gas chromatography
- High-pressure liquid chromatography
- Modification of the properties by derivatisation
- Methods of quantification
- Semi-preparative HPLC
- UPLC
- Enantiomeric separations

Aims

At the end of this learning unit, the student is able to :

a. Contribution de l¿activité au référentiel AA (AA du programme)

The course contributes to the following learning outcomes : 1.3, 1.4, 3.3, 6.2 and 6.8 (BIR program)

b. Formulation spécifique pour cette activité des AA du programme (maximum 10)

At the end of this course, the student will be able

to select the best technique to get rid of all major co-constituents,
to optimize the resolution of the GC/HPLC chromatograms,
to choose the best detector in terms of sensibility and selectivity,
to accurately quantify the analytes (use of internal standards, external standards, standard addition, isotopic dilution ..)
and to extrapolate the concepts to isolate them by (semi)preparative HPLC or GC.

The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.

Content

Teaching methods

Half of the courses consist in magistral lectures, the other part being a reflection (groups of students) around published experimental protocols. The students have also the opportunity to apply a few number of experimental protocols at the laboratory.

Evaluation methods

Written examination for the theoretical part. 3 Oral communications (in English) are presented during the seminars (discussion of experimental protocols). The experimental know-how and the attitude are evaluated throughout practical classes (laboratory report).

Online resources

Icampus

Bibliography

Le cours ne fait appel à aucun support particulier qui serait payant et jugé obligatoire

Faculty or entity

AGRO

Programmes / formations proposant cette unité d'enseignement (UE)

Title of the programme

Sigle

Credits

Prerequisites

Aims

Bachelor in Bioengineering

BIR1BA