Teacher(s)

Morsomme Pierre; Soumillion Patrice;

Language

French

Main themes

The course will be divided into two parts:1. Structural biochemistry:- patterns and forms in protein structures (observation, manipulation, description, classification)- principles of protein folding - domains and assemblies (modular nature of proteins, multi-protein complexes)- bioinformatics in structural biology- interactions between biomolecules (methods and characterization)2. Enzymology- principles of enzyme catalysis (Michaelis Menten, steady state kinetics, reaction schemes)- the basics of chemical catalysis by proteins (catalysis by amino acids lateral chains, active site complementarity, entropic catalysis, transition state stabilisation)- control of enzyme activity (inhibition, activation, cooperativity, allosterism, environmental effects)

Content

About 24 hours will be dedicated to lectures with the help of PowerPoint slides. 6 hours will be organized in computer room for exercises or guided tours of various websites. Content: 1. Amino acids side chains: - hydrophobicity/hydrophilicity - electrostatic and acido-basic properties - nucleophilicity - redox properties 2. Structural biochemistry: - biomolecule structures and interactions: non covalent driving forces and quantitative aspects - thermodynamical and chemical stability of proteins - protein folding : from molecular mechanisms to conformational diseases - observation, manipulation, visualisation, description and classification of three-dimensional structures (in computer room) 3. Enzymology - principles of enzyme catalysis (Michaelis Menten, steady state kinetics, reaction schemes) - the basics of chemical catalysis by proteins (catalysis by amino acids lateral chains, active site complementarity, entropic catalysis, transition state stabilisation) - cofactors and coenzymes chemistries - control of enzyme activity (inhibition, activation, cooperativity, allosterism, environmental effects) - numerical simulation of enzymatic catalysis (in computer room)

Other information

Precursory courses: Basics in biochemistry (e.g. Elements of biochemistry CHM1271) Evaluation: Written and oral examination Support: PowerPoint slides

Faculty or entity

BIOL