Atelier d'histologie [ WSBIM1204 ]

Histology of the investigated tissues needs to be known.

Theory about morphological methods used to investigate biological methods

and practical training to address an exemplative problem in one week

·           To gain theoretical and practical knowledge of histological and immunohistological techniques, and theoretical notions of in situ hybridization.

·           To solve a biological problem by using morphological methods.

Evaluation will be made by oral examination with presentation of the histological section at the microscope and discussion about the report.

·           Theory :

Histological techniques : frozen sections, paraffin sections, synthetic resin embedding

Immunohistochemical techniques

In situ hybridization

·           Practical :

Mouse dissection and tissues sampling

Preparation of buffers and fixative solutions

Tissue freezing or fixation in formalin

Frozen sections (demonstration)

Immunohistochemistry on frozen sections (demonstration)

Paraffin embedding

Paraffin sections

H&E and PAS staining

Immunohistochemistry on paraffin sections

Supervised microscopic analysis (video screening)

Students will be supervised by PhD students during the training.

Students will have to make a report of the study, resembling a scientific article. They will be paired for this report (one student investigating the control animal, the other one the test animal).