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Atelier d'histologie [ WSBIM1204 ]


2.0 crédits ECTS  30.0 h   2q 

Teacher(s) Guiot Yves ; Marbaix Etienne (coordinator) ; Many Marie-Christine ;
Language French
Place
of the course
Bruxelles Woluwe
Prerequisites

Histology of the investigated tissues needs to be known.

Main themes

Theory about morphological methods used to investigate biological methods

and practical training to address an exemplative problem in one week

Aims

·           To gain theoretical and practical knowledge of histological and immunohistological techniques, and theoretical notions of in situ hybridization.

·           To solve a biological problem by using morphological methods.

Evaluation methods

Evaluation will be made by oral examination with presentation of the histological section at the microscope and discussion about the report.

Content

·           Theory :

Histological techniques : frozen sections, paraffin sections, synthetic resin embedding

Immunohistochemical techniques

In situ hybridization

 

·           Practical :

Mouse dissection and tissues sampling

Preparation of buffers and fixative solutions

Tissue freezing or fixation in formalin

Frozen sections (demonstration)

Immunohistochemistry on frozen sections (demonstration)

Paraffin embedding

Paraffin sections

H&E and PAS staining

Immunohistochemistry on paraffin sections

Supervised microscopic analysis (video screening)

Other information

Students will be supervised by PhD students during the training.

Students will have to make a report of the study, resembling a scientific article. They will be paired for this report (one student investigating the control animal, the other one the test animal).

Cycle et année
d'étude
> Bachelor in Biomedicine
Faculty or entity
in charge
> SBIM


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