Applied biotechnology [ LBIRA2102 ]

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Basic knowledge in Genetics (LBIR1322), Biochemistry (LBIR1321), Microbiology (LBIR1323), Animal Physiology (BIR1324) and Plant Physiology (BIO1341)

General principles related to cell culture, cell modification and organism regeneration in animals, plants and microorganisms.

a. Contribution of the activity to the LO (LO from the program)

M1.1, M2.1, M3.1, M5.6, M6.1, M7.1

b. LO from the program specific to this activity

At the term of the activity, the student will be able to:

- understand the main biotechnologies related to animals, plants and microorganisms that are used in the context of production and/or improvement,

- classify the main applied biotechnologies related to animals, plants and microorganisms,

- describe clearly and simply the major steps of a given biotechnology,

- understand the dynamics of biotechnological evolution beyond the conventional boundaries of life domains,

- objectively address questions downstream those technologies (economy, nutrition, health, society) by using the fundamentals and the context of biotechnological innovation.

Written examination

Lectures

Seminars ' analysis and presentation of scientific papers

Lab visits

-       Basic knowledge of cell culture, manipulation and conservation.

1.    Microorganisms (microbial kinetics, strains preservation)

2.    Animals (stem cells, in vitro production of embryos and related techniques, cryobanking)

3.    Plants (totipotency, in vitro culture, protoplasts culture, doubled haploids, cell growth regulation and development)

-       Principles of cell modification (transformation, transgenesis, mutagenesis, heterologous/homologous recombination, vectors)

-       DNA technologies, isolation and cloning, gene construction, genomics

-       Organisms regeneration from modified cells (animal and plant cloning, selection, markers, protoplast fusion, in vitro culture)

Those topics are separately addressed for plants, animals and microorganisms. The comparison between the three domains is addressed by means of seminars presented by the students.

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