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Structure, fonction et régulation des protéines (1re partie) [ WSBIM2115 ]


4.0 crédits ECTS  30.0 h   1q 

Teacher(s) Damblon Christian ; Rider Mark (coordinator) ; Collet Jean-François ; Bertrand Luc ; Opperdoes Frederik ;
Language French
Place
of the course
Bruxelles Woluwe
Prerequisites

Good background in chemistry, physics and biochemistry. Basic computer skills (including use of search engines and data base searching).

 

Main themes

Methods of expression, purification an in vitro renaturation of proteins (5h)

Protein sequencing (2h)

Bioinformatic analysis of proteins (homology searches, alignments, phylogenetic studies, motif and domain searching, structure modelling) (10h)

Structure determination by NMR, crystal structures by X-ray diffraction in relation to function (3h)

Enzymology (thermodynamics, pre- and steady state kinetics, calculation of kinetic parameters, ligand binding and allosteric enzymes, site-directed mutagenesis, theory of metabolic control) (10 h)

 

Aims

To provide Masters students in Biomedical Sciences with the necessary competence to study:

-          techniques of overexpression/purification and structural analysis of proteins

-          structure-function relationships in proteins

-          physiological roles of enzymes

Evaluation methods

Written exam

Teaching methods

Formal lectures, background reading

Content

Enzymology, purification, sequencing, structure determination and bioinformatic analysis of proteins.

Bibliography

Support: PDF files of Power Point présentations will be made available to the students on iCampus.

Other information

Teaching of the course material will be in French but Power Point files will be mostly in English

Cycle et année
d'étude
> Master [120] in Biomedicine
> Master [240] in Medecine
> Master [60] in Biomedicine
> Master [120] in Public Health
> Master [120] in Statistics: Biostatistics
Faculty or entity
in charge
> SBIM


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