Analyse instrumentale (techniques chromatographiques et 10h de travaux pratiques) [ WFARM1312T ]

general chemistry ; organic chemistry ; introduction to the analytical chemistry

The teacher(s) will discuss the different kinds of spectroscopic techniques (UV, molecular fluorescence, atomic spectroscopy') ; and will then focus on the separation techniques such as HPLC and GC. They will also discuss the detectors that are used to detect the analytes following their separation (UV, FID, MS').

At the end of the activity the student will be able to

• Differentiate the different spectroscopic techniques (type of interaction with the light, nature of the measured signal, ')
• Describe the different separation techniques that have been discussed
• Explain the consequences of a change in the experimental conditions of a separation on the result of the separation.
• Propose, based on the elements discussed during the course, the optimal method allowing the quantification of a given analyte.

a written exam spanning from theoretical aspects to exercise resolution

WFARM1313 (practical training in instrumental analysis) allows to approach the theoretical notions in a more practical way.

• Spectroscopic techniques
  • UV-Visible
  • Molecular fluorescence
  • Atomic spectroscopy
• Introduction to the analytical separations
• Electrophoretic methods
• Liquid chromatograpy
• Gaz chromatography
• Introduction to the mass spectrometry