Structure, fonction et régulation des protéines (1re partie) [ WSBIM2115 ]

Good background in chemistry, physics and biochemistry. Basic computer skills (including use of search engines and data base searching).

Methods of expression, purification an in vitro renaturation of proteins (5h)

Protein sequencing (2h)

Bioinformatic analysis of proteins (homology searches, alignments, phylogenetic studies, motif and domain searching, structure modelling) (10h)

Structure determination by NMR, crystal structures by X-ray diffraction in relation to function (3h)

Enzymology (thermodynamics, pre- and steady state kinetics, calculation of kinetic parameters, ligand binding and allosteric enzymes, site-directed mutagenesis, theory of metabolic control) (10 h)

To provide Masters students in Biomedical Sciences with the necessary competence to study:

-          techniques of overexpression/purification and structural analysis of proteins

-          structure-function relationships in proteins

-          physiological roles of enzymes

Written exam

Formal lectures, background reading

Enzymology, purification, sequencing, structure determination and bioinformatic analysis of proteins.

Support: PDF files of Power Point présentations will be made available to the students on iCampus.

Teaching of the course material will be in French but Power Point files will be mostly in English