Methodolgy of cell and molecular biology [ WSBIM1211 ]
3.0 crédits ECTS
22.5 h
2q
| Teacher(s) |
Pierreux Christophe ;
Courtoy Pierre (coordinator) ;
Van Schaftingen Emile ;
Collet Jean-François ;
|
| Language |
French
|
Place
of the course |
Bruxelles Woluwe
|
| Main themes |
Methodologies currently discussed are (1) principles and methods of protein purification, including the calculation of a purification table; (2) principles, applications and safety rules in the use of radioactivity as a tool in biochemistry and cell biology; (3) principles and applications of cell culture; (4) the physical basis, methods, potentials and limitations of analytical subcellular fractionation ; and (5) morphological methods, with emphasis on molecular tracking in fixed and living cells
|
| Aims |
To get a critical grasp on a few essential methodologies in cell and molecular biology, on which teachers have a special expertise. The course primarily aims at the understanding of basic principles and inherent limitations, so as to help students in selecting the most appropriate approach to address a specific question. This teaching further demands the quantitative analysis of the observations and the differentiation between warranted and unjustified conclusions from a particular experiment
|
| Content |
protein purification; radioactivity as a tool in research; cell culture; subcellular fractionation; molecular tracking in fixed and living cells.
|
| Other information |
Prerequisite : cell biology and general biochemistry
Calendar : second quadrimester, each Thursday, from 14:00 to 15 :45 at ICP 75-1.
Individual support : printed notes
Examination : quantitative analysis of new data obtained with one or several of described methods and usually presented in English; testing of their understanding
|
Cycle et année
d'étude |
> Preparatory year for Master in Biomedicine
> Bachelor in Biomedicine
|
Faculty or entity
in charge |
> SBIM
|
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