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Methodolgy of cell and molecular biology [ WSBIM1211 ]


3.0 crédits ECTS  22.5 h   2q 

Teacher(s) Collet Jean-François ; Pierreux Christophe ; Courtoy Pierre (coordinator) ; Van Schaftingen Emile ;
Language French
Place
of the course
Bruxelles Woluwe
Main themes Methodologies currently discussed are (1) principles and methods of protein purification, including the calculation of a purification table; (2) principles, applications and safety rules in the use of radioactivity as a tool in biochemistry and cell biology; (3) principles and applications of cell culture; (4) the physical basis, methods, potentials and limitations of analytical subcellular fractionation ; and (5) morphological methods, with emphasis on molecular tracking in fixed and living cells
Aims To get a critical grasp on a few essential methodologies in cell and molecular biology, on which teachers have a special expertise. The course primarily aims at the understanding of basic principles and inherent limitations, so as to help students in selecting the most appropriate approach to address a specific question. This teaching further demands the quantitative analysis of the observations and the differentiation between warranted and unjustified conclusions from a particular experiment
Content protein purification; radioactivity as a tool in research; cell culture; subcellular fractionation; molecular tracking in fixed and living cells.
Other information Prerequisite : cell biology and general biochemistry Calendar : second quadrimester, each Thursday, from 14:00 to 15 :45 at ICP 75-1. Individual support : printed notes Examination : quantitative analysis of new data obtained with one or several of described methods and usually presented in English; testing of their understanding
Cycle et année
d'étude
> Bachelor in Biomedicine
> Preparatory year for Master in Biomedicine
Faculty or entity
in charge
> SBIM


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