UCL - Introduction to protein biotechnology

[CHM2170]

[22.5h + 7.5h] 3 credits

Teacher(s): Pierre Morsomme, Patrice Soumillion

Language: French

Level: Second

Aims
Main themes
Other information (prerequisite, evaluation (assessment methods), course materials recommended readings, ...)

Aims

The student will get to know the field of protein biotechnology for which the interactions with chemistry are continuously growing, especially in bio-pharmacy. He will learn the notions of molecular biology and genetic engineering that are useful with regard to the production and improvement of proteins. Another objective is also to acquire the vocabulary associated with these notions so that the student will later be able to interact with the experts of that field.

Main themes

1. Gene manipulation: recovery, cloning, modification, transfer and characterisation.
2. Gene expression: vectors, expression in bacteria, yeasts, plants, insect and mammalian cells, production of monoclonal antibodies.
3. Protein improvement: genetic engineering, directed evolution and chemical stabilisation.
All the underlying techniques will be briefly explained.

Other information (prerequisite, evaluation (assessment methods), course materials recommended readings, ...)

The course could be partly or totally delivered by an invited lecturer.