UCL - Training course in cell biology

[SBIM1293]

[30h] 2 credits

Teacher(s): Pascal Kienlen-Campard(coord.), Jean-Christophe Renauld

Language: French

Level: First

Aims
Main themes
Content and teaching methods
Other information (prerequisite, evaluation (assessment methods), course materials recommended readings, ...)

Aims

The aim is to learn the basic techniques of cell biology:
-cell culture and propagation ;
-morphological and microscopic examination of the cells ;
-analysis of cell proliferation and cell survival,
-introduction to the analysis of cell populations by FACS (Fuorescence-Associated Cell Sorter).
Our aim is also to train students to write a laboratory notebook and a training course report.

Main themes

-culture of cell lines in sterile conditions ;
-cell analysis by fluorescent microscopy ;
-biochemical assays (proteins, cell proliferation and cell survival assays) ;
-introduction to FACS analysis and study of the characteristic profile of selected cell populations.

Content and teaching methods

This training course takes place during 5 consecutive afternoons in two research laboratories of the Faculty. Students will work in small groups (usually 2 students) under the supervision of a research scientist.

Other information (prerequisite, evaluation (assessment methods), course materials recommended readings, ...)

Prérequisites : those of Bac2.
Links : This training course requires knowledges from the practical courses of biology (Bac1, MD1107, General Biology) and prepares to the further laboratory training courses (SBIM9212, Stage de laboratoire)
Assessment : continuous and on the basis of the training course report.
Supervision : an assistant (with the help of other members of the research group).