UCL - Complements of spectroscopy

[CHM2151]

[22.5h + 7.5h] 3 credits

Teacher(s):

Language: English

Level: Second

Aims
Main themes
Content and teaching methods
Other information (prerequisite, evaluation (assessment methods), course materials recommended readings, ...)

Aims

This course covers technical aspects of mass spectrometry and interpretation of spectral data.

Main themes

1. Basic definitions
2. Ionization modes
3. Analyzers
4. Chromatographic couplings
5. Spectral data interpretation
6. Introduction to the identification and sequencing of proteins and peptides by mass spectrometry.

Content and teaching methods

After a brief survey of the basic definitions, the various mode of ionization (EI, CI, FAB, ESI, APCI, APPI, DESI, DAPCI, DAPPI, EESI, thermospray, ASAP) will be described in details. The different analyzers (Quad, triple Quad, Trappes, TOF, Orbitrap, FTICR) will be presented together with their possible scanning modes and their combinations. Couplings with Gc and HPLC will be presented. The interpretation of spectral data will first emphasize the differences between low resolution and low accuracy versus high resolution and high accuracy data. The importance of the isotopic cluster will be demonstrated. The principal rules of fragmentation of radical-cations will be presented together with some basic rules for the fragmentation of ions with even-number of electrons. Selected examples and exercises will be explicitly studied in this part of the course. At the end of the course, a short introduction to the use of mass spectrometry for the analysis of proteins and peptides and for the analysis of non-covalente species will be given.

Other information (prerequisite, evaluation (assessment methods), course materials recommended readings, ...)

Prerequisite:
- Basic knowledge of chemistry and physics
- CHM1251C course.